Creating 3D model from PDB file

1. Download PDB file from PDB website

2. Load EMAN2 software:

module load eman2

3. Then run:

$ e2pdb2mrc.py inputfile.pdb outputfile.mrc --apix=[pixel size] --box=[box size] --center

Where:

• --apix is the pixel of the resulting 3D model
• --box is the box size of the resulting 3D model in pixels
• --center makes sure that the model is centered in the new 3D volume